Home > Faculties > Chung, In Sik

Chung, In Sik
Professor, Biotechnology Lab.
Tel: 82-31-201-2436(office), 031-201-2139(lab)
E-mail: ischung@khu.ac.kr
BS, Seoul National Univ., Food Technology (1972-1976)
Ph.D., Auburn Univ., Biotechnology (1980-1986)
Visiting Research Professor, Cornell Univ. (1991-1992)
Visiting Research Professor, Ohio State Univ. (2000-2001)
Editor, Plant Cell Reports (Springer) (2000-2006)
Board of Advisors, American Biographical Institute (2006-present)
Professor, Kyung Hee Univ., Genetic Engineering (1987-present)
Professor and Chair, Kyung Hee Univ., Graduate School of Biotechnology (2006-present)

We are presently conducting research on several projects related to medical and plant biotechnology. In the field of medical biotechnology, we are investigating the molecular mechanisms of tumstatin and canstatin in squamous cell carcinoma (SCC) cells using genomics and molecular biology methods. We are also analyzing the effects of tumstatin and canstatin in animal models for SCC. We also plan to develop molecular target therapy for SCC using tumstatin, canstatin and other angiogenesis inhibitor in future clinical trials.

In the field of plant biotechnology, we are investigating the genetic engineering of plants for recombinant protein production with a major focus on vaccines and therapeutics, while simultaneously working on the metabolic engineering of phytochemical biosynthesis pathway in plants.

-Investigation of molecular mechanisms of tumstatin and canstatin in AT-84 and SCCⅦ cells utilizing genomics and molecular biology methods
-Molecular target therapy for SCC using tumstatin, canstatin and other angiogenesis inhibitor
-Development of edible vaccines against human hepatitis A, influenza and rotavirus diseases
-Bioreactor design and scale-up for the production of biopharmaceuticals

1. Hwang-Bo J, Yoo KH, Jeong HS, Chung IS (2009) Recombinant canstatin inhibits tumor growth in an orthotopic AT-84 oral squamous cell carcinoma model. Biotechnol. Lett. October 17 Online Publication.

2. Lee JM, Lee HH, Hwang-Bo J, Shon DH, Kim W, Chung IS (2008) Expression and immunogenicity of recombinant VP1 of human hepatitis A virus. Biotechnol. Appl. Biochem. 53:101-109.

3. Chung IS, Son YI, Baek CH, Cho JK, Jeong HS (2008) Peritumor injections of purified tumstatin delay tumor growth and lymphatic metastasis in an orthotopic oral squamous cell carcinoma model. Oral Oncology 44:1118-1126.

4. Park JH, Hwang IS, Lee JM, Park YM, Park CH, Chung IS (2008) Functional expression of recombinant human ribonuclease/angiogenin inhibitor in stably transformed Drosophila melanogaster S2 cells. Cytotechnology 57:93-99.

5. Kim NY, Kim HG, Kim YH, Chung IS, Yang JM (2008) Expression and characterization of human N-acetylglucosaminyltransferases and alpha2,3-Sialyltransferase in insect cells for in vitro glycosylation of recombinant erythropoietin. J. Microbiol. Biotechnol. 18:383-391.

6. Chang KH, Lee JM, Hwang-Bo J, Yoo KH, Shon BH, Yang JM, Chung IS (2007) Expression of recombinant cyclooxygenase 1 in Drosophila melanogaster S2 cells transformed with human β1,4-galactosyltransferase and Galβ1,4GlcNAcα2,6-sialytransferase. Biotechnol. Lett. 29:1803-1809.

7. Kim KI, Sunter G, Bisaro DM, Chung IS (2007) Improved expression of recombinant GFP using a relicating vector based on Beet curly top virus in leaf-disks and infiltrated Nicotina benthamiana leaves. Plant Mol. Biol. 64:103-112.

8. Lee JM, Hwang-Bo J, Shon BH, Chung IS (2007) Functional expression of recombinant canstatin in stably transformed Drosophila melanogaster S2 cells. Protein Expr. Purif. 52:258-264.

9. Lee DY, Song MC, Yoo KH, Bang MH, Chung IS, Kim SH, Kim DK, Kwon BM, Jeong TS, Park MH, Baek NI (2007) Lignans from the fruits of Cornus kousa Brug. and their cytotoxic effects on human cancer cell lines. Arch. Pharm. Res. 30:402-407.

10. Chang KH, Baek NI, Yang JM, Lee JM, Hwang-Bo J, Chung IS (2005) Expression and characterization of recombinant β-secretase from Trichoplusia ni BTI Tn5B1-4 cells transformed with cDNAs encoding human β1,4-galactosyltransferase and Gal β1,4-GlcNAcα2,6-sialytransferase. Protein Expr. Purif. 44:87-93.

11. Chang KH, Yang JM, Chun HO, Chung IS (2005) Enhanced activity of recombinant β-secretase from Drosophila melanogaster S2 cells transformed with cDNAs encoding humam β1,4-galactosyltransferase and Galβ1,4-GlcNAcα2,6-sialytransferase. Journal of Biotechnology 116:359-367.

12. Kim WY, Chung IS, Lee YH, Kim HJ (2005) Production method of recombinant rotavirus structural proteins and vaccine composition. United States Patent #6867353.

13. Chang KH, Lee JM, Jeon HK, Chung IS (2004) Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells. Protein Expr. Purif. 35:69-75.

14. Hong SH, Kim KI, Chung HY, Kim YJ, Sunter G, Bisaro DM, Chung IS (2004) Expression of recombinant endostatin in Agrobacterium-inoculated leaf-disks of Nicotiana tabacum var. xanthi. Biotechnol. Lett. 26:1433-1439.

15. Chang KH, Lee JM, Jeon HK, Chung IS (2004) Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells. Protein Expr. Purif. 35:69-75.

16. Kim HG, Yang YC, Lee YC, Do SI, Chung IS, Yang JM (2003) High-level expression of human glycosyltransferase in insect cells as biochemically active form. Biochem. Biophys. Res. Comm. 305:488-493.

17. Jeon HK, Chang KH, Kim KI, Chung IS (2003) Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells. Biotechnol. Lett. 25:185-189.

18. Chang KH, Chung IS, Park CH (2003) Concentrating rotavirus and recombinant-enhanced green fluorescent protein from E. coli using a pH-sensitive hydorgel. Biotechnol. Lett. 25:335-338.

19. Chang KH, Park JH, Lee YH, Kim JH, Chun HO, Kim JH, Chung IS (2002) Dimethylsulfoxide and sodium butyrate enhance the production of recombinant cyclooxygenase2 in stably transformed Drosophila melanogaster S2 cells. Biotechnol. Lett. 24:1353-1359.

20. Park JH, Chang KH, Lee YH, Kim HY, Yang JM, Chung IS (2002) Production of recombinant rotavirus capsid protein VP7 from stably transformed Drosophila melanogaster S2 cells. J. Microbiol. Biotechnol. 12:563-568.